EXPERIMENTAL PROTOCOL FOR SYSTEMIC ANF
                INFUSION STUDIES IN THE NORMAL RAT

1) WEIGH RAT 
2) ANESTHETIZE 
3) CANULATE ONE CAROTID ARTERY, ONE JUGULAR, ONE FEMERAL ARTERY AND THE 
        BLADDER.
4) PREPARE INULIN AND PAH  INFUSION SOLUTION IN ADVANCE.
        TOTAL VOLUME = 30ML OF SOLUTION (NORMAL SALINE) ONE ADDS TO THESE
        30ML OF THE FOLLOWING AMOUNTS OF INULIN (IN MG) = 180 * GM OF RAT/100
                                         PAH    (IN MG) =  30 * GM/100

        AFTER DESOLVING  INULIN AND PAH IN SALINE SOLUTION AND COOLING TO ROOM 
        TEMPERATURE YOU CAN START INFUSION AT 33.34 ul / min. WAIT 1 TO 1.5
        HRS FOR EQUILIBRIUM OF PAH AND INULIN BEFORE BEGINNING CLEARANCE
        STUDY.
5) TAKE BASAL (30') URINE AND BLOOD SAMPLE
6) ANF BOLUS I.V. = 2ug/kg
        ANF STOCK SOLUTION = 10ML OF NORMAL SALINE; ADD 60.8 ul OF ANF STOCK
         TO GET 1.216 ug/ml STOCK. EXAMPLE FOR  A 150 GM RAT YOU GIVE 246.7 ul
         OF STOCK.
7) BLOOD PRESSURE MUST BE MEASURED BEFORE AND DURING I.V. BOLUS OF ANF.
8) A CONSTANT INFUSION OF ANF ( 0.3 ug/kg/min) IS STARTED, USING THE STOCK
        SOLUTION .
        EXAMPLE. FOR  A 150 gm RAT YOU NEED  TO INFUSE 0.0345 ug/min OR
                0.037 ml/min  I.V. OF ANF STOCK SOLUTION. 
9) A CLEARANCE PERIOD IS BEGUN WITH B0LUS ADMINISTRATION AND CONTINUED FOR 
        30 MIN.
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        DAY BEFORE THE EXPERIMENT ONE SHOULD PREWEIGH EPPENDORF TUBES FOR 
URINE SAMPLES.