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BUFFER PREPARATION
2-28-89
BUFFER PREPERATION
CONC. MW mM
NaCl 6.136g/L 58.44 105
KCl 0.2728g/L 74.55 5
MgSO4-7H2O 0.1973g/L 246.48 0.8
CaCl2-2H2O 0.3675g/L 147.03 2.5
NaH2PO4-H2O 0.3218g/L 137.99 1.2
NaHCO3 2.100g/L 84.00 25
Glucose (Dextrose) 0.9025g/L 180.20 5
MAKE pH to 7.4 w/ 2N HCl
INULIN PAH PREPERATION
Make up in 0.9% NaCl
Inulin 13mg/cc or 1.3g/100cc
PAH 15mg/cc or 0.5 g/100
Take approximately 1/3 total volume of saline and dissolve inulin. Bring
mixture to a boil. Turn off hot plate and leave beaker and solution to cool.
After a few minutes of cooling add PAH and stirrer and more diluent.
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BUFFER PREPARATION