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                                                2-28-89
                         BUFFER PREPERATION

                               CONC.             MW               mM
NaCl                         6.136g/L          58.44             105
KCl                          0.2728g/L         74.55               5
MgSO4-7H2O                   0.1973g/L        246.48             0.8
CaCl2-2H2O                   0.3675g/L        147.03             2.5
NaH2PO4-H2O                  0.3218g/L        137.99             1.2
NaHCO3                       2.100g/L          84.00             25
Glucose (Dextrose)           0.9025g/L        180.20              5
                                                
                                                     MAKE pH to 7.4 w/ 2N HCl


                         INULIN PAH PREPERATION


Make up in 0.9% NaCl 

        Inulin 13mg/cc or 1.3g/100cc

        PAH 15mg/cc or 0.5 g/100

Take approximately 1/3 total volume of saline and dissolve inulin. Bring 
mixture to a boil. Turn off hot plate and leave beaker and solution to cool.
After a few minutes of cooling add PAH and stirrer and more diluent.


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