STANDARD PROTEIN ASSAY - LOWREY

STOCK SOLUTIONS
        SOL A) 20g Na2CO3 1 LITER OF 0.1 N NaOH
        SOL B) 2% NA TARTRATE
        SOL C) 1% Cu SO4 - 5 H2O
        SOL D) 2N PHENOL REAGENT (FOLIN CIOCALTEUS)

WORKING REAGENTS 
        1) BASE REAGENT
                1 ml SOL B
               +1 ml SOL C
                ----------
                QS TO 100 ml WITH SOLU A

        2) PHENOL REAGENT (1N)
                1 ml SOL D
               +1 ml H2O
                ----------
                2 ml 1N WORKING PHENOL REAGENT

STANDARD CURVES
        REFERENCE STOCK = 10g/dl (VALUE STATED ON BOTTLE)
        USING REFERENCE STOCK TO MAKE EXPANDED PROTEIN CURVE REQUIRES A 1/10 
        DILUTION TO MAKE YOUR WORKING REFERENCE. EXAMPLE  1ml REFERENCE STOCK
        [10 g/dl 100 mg/ML] DILUTE TO 10 ml TOTAL VOLUME WITH .9N NaCl
        WORKING REFERENCE CONC. (10 mg/ml).